Abnormal islet sphingolipid metabolism in type 1 diabetes.

ABSTRACT:

Aims/hypothesis

Sphingolipids play important roles in beta cell physiology, by regulating proinsulin folding and insulin secretion and in controlling apoptosis, as studied in animal models and cell cultures. Here we investigate whether sphingolipid metabolism may contribute to the pathogenesis of human type 1 diabetes and whether increasing the levels of the sphingolipid sulfatide would prevent models of diabetes in NOD mice.

Methods

We examined the amount and distribution of sulfatide in human pancreatic islets by immunohistochemistry, immunofluorescence and electron microscopy. Transcriptional analysis was used to evaluate expression of sphingolipid-related genes in isolated human islets. Genome-wide association studies (GWAS) and a T cell proliferation assay were used to

SUBMITTER: Holm LJ 

PROVIDER: S-EPMC6445476 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature