PAN-INTACT enables direct isolation of lineage-specific nuclei from fibrous tissues.

ABSTRACT: Recent studies have highlighted the extraordinary cell type diversity that exists within mammalian organs, yet the molecular drivers of such heterogeneity remain elusive. To address this issue, much attention has been focused on profiling the transcriptome and epigenome of individual cell types. However, standard cell type isolation methods based on surface or fluorescent markers remain problematic for cells residing within organs with significant connective tissue. Since the nucleus contains both genomic and transcriptomic information, the isolation of nuclei tagged in specific cell types (INTACT) method provides an attractive solution. Although INTACT has been successfully applied to plants, flies, zebrafish, frogs, and mouse brain and adipose tissue, broad use across mammalian organs re

SUBMITTER: Bhattacharyya S 

PROVIDER: S-EPMC6445515 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature