Comprehensive B Cell Phenotyping Profile for Chronic Graft-versus-Host Disease Diagnosis.

ABSTRACT: Previous studies have reported single B cell-related chronic graft-versus-host disease diagnostic (cGVHD) biomarkers, such as B cell-activating factor (BAFF), CD21^low, and immature B cells, but research on the performance of biomarker combinations and the covariate effect of steroids is lacking. The primary objective of this study was to determine the most accurate combination of B cell populations using cell surface staining flow cytometry in an independent cohort of patients with cGVHD. Secondary objectives included assessing the effect of corticosteroid use at sample collection on the makeup and accuracy of the diagnostic panel and identifying the mechanism underlying low surface expression of BAFF receptor (BAFF-R) on B cells in cGVHD. Flow cytometry analysis was performed i

SUBMITTER: Rozmus J 

PROVIDER: S-EPMC6445755 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature