Differential expression of interferon-induced genes and other tissue-based biomarkers in acute graft-versus-host disease vs. lupus erythematosus in skin.

ABSTRACT:

Background

In both acute graft-versus-host disease (GVHD) and lupus erythematosus (LE), the patient's own tissues are subjected to immunological assault via complex mechanisms influenced by interferon (IFN) and other cytokines. Although not typically confused clinically, these entities have overlapping histopathological findings in the skin.

Aim

To assess whether GVHD can be differentiated from LE using molecular methods on skin specimens.

Methods

We developed a quantitative reverse transcription PCR assay based on previously identified tissue-based biomarkers of cutaneous GVHD, and compared gene expression in GVHD with that in LE.

Results

Both entities showed robust expression of IFN-induced genes and of genes encoding proteins involved in antigen presentation

SUBMITTER: Lehman JS 

PROVIDER: S-EPMC6445793 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature