Development of event-specific qPCR detection methods for genetically modified alfalfa events J101, J163 and KK179.

ABSTRACT: Genetically modified alfalfa is authorized for cultivation in several countries since 2005. On the other hand, cultivation in or export to the European Union is not allowed and thus neither certified reference material nor official event-specific detection methods are available. Therefore, based on patent sequence information, event-specific real-time PCR detection methods targeting the junction sequence of the alfalfa genome and the transgenic insert of the respective events J101, J163 and KK179 were developed. Newly developed plasmids were used as reference material for assay optimization and in-house validation. Plasmid standards were quantified using digital droplet PCR and LOD95%, PCR efficiency, robustness and specificity of the assays were determined using real-time PCR. A LOD95% of

SUBMITTER: Guertler P 

PROVIDER: S-EPMC6446038 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature