RNA-sequencing across three matched tissues reveals shared and tissue-specific gene expression and pathway signatures of COPD.

ABSTRACT:

Background

Multiple gene expression studies have been performed separately in peripheral blood, lung, and airway tissues to study COPD. We performed RNA-sequencing gene expression profiling of large-airway epithelium, alveolar macrophage and peripheral blood samples from the same subset of COPD cases and controls from the COPDGene study who underwent bronchoscopy at a single center. Using statistical and gene set enrichment approaches, we sought to improve the understanding of COPD by studying gene sets and pathways across these tissues, beyond the individual genomic determinants.

Methods

We performed differential expression analysis using RNA-seq data obtained from 63 samples from 21 COPD cases and controls (includes four non-smokers) via the R package DESeq2. We tested ass

SUBMITTER: Morrow JD 

PROVIDER: S-EPMC6446359 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature