Differential effects of the Akt inhibitor MK-2206 on migration and radiation sensitivity of glioblastoma cells.

ABSTRACT:

Background

Most tumor cells show aberrantly activated Akt which leads to increased cell survival and resistance to cancer radiotherapy. Therefore, targeting Akt can be a promising strategy for radiosensitization. Here, we explore the impact of the Akt inhibitor MK-2206 alone and in combination with the dual PI3K and mTOR inhibitor PI-103 on the radiation sensitivity of glioblastoma cells. In addition, we examine migration of drug-treated cells.

Methods

Using single-cell tracking and wound healing migration tests, colony-forming assay, Western blotting, flow cytometry and electrorotation we examined the effects of MK-2206 and PI-103 and/or irradiation on the migration, radiation sensitivity, expression of several marker proteins, DNA damage, cell cycle progression and the pla

SUBMITTER: Djuzenova CS 

PROVIDER: S-EPMC6446411 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature