Glycan distribution and density in native skin's stratum corneum.

ABSTRACT:

Background

The glycosylation of proteins on the surface of corneocytes is believed to play an important role in cellular adhesion in the stratum corneum (SC) of human skin. Mapping with accuracy the localization of glycans on the surface of corneocytes through traditional methods of immunohistochemistry and electron microscopy remains a challenging task as both approaches lack enough resolution or need to be performed in high vacuum conditions.

Materials and methods

We used an advanced mode of atomic force microscope (AFM), with simultaneous topography and recognition imaging to investigate the distribution of glycans on native (no chemical preparation) stripped samples of human SC. The AFM cantilever tips were functionalized with anti-heparan sulfate antibody and the lectin

SUBMITTER: Danzberger J 

PROVIDER: S-EPMC6446803 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature