Sulfur Dioxide Activates Cl^-/HCO₃ ^- Exchanger via Sulphenylating AE2 to Reduce Intracellular pH in Vascular Smooth Muscle Cells.

ABSTRACT: Sulfur dioxide (SO₂) is a colorless and irritating gas. Recent studies indicate that SO₂ acts as the gas signal molecule and inhibits vascular smooth muscle cell (VSMC) proliferation. Cell proliferation depends on intracellular pH (pH_i). Transmembrane cystein mutation of Na⁺- independent Cl^-/HCO₃ ^- exchanger (anion exchanger, AE) affects pH_i. However, whether SO₂ inhibits VSMC proliferation by reducing pH_i is still unknown. Here, we investigated whether SO₂ reduced pH_i to inhibit the proliferation of VSMCs and explore its molecular mechanisms. Within a range of 50-200 μM, SO₂ was found to lower the pH_i in VSMCs. Concurrently, NH₄

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6446831 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature