Cerebellar ataxia disease-associated Snx14 promotes lipid droplet growth at ER-droplet contacts.

ABSTRACT: Lipid droplets (LDs) are nutrient reservoirs used by cells to maintain homeostasis. Nascent droplets form on the endoplasmic reticulum (ER) and grow following an influx of exogenous fatty acids (FAs). The budding of LDs requires extensive ER-LD crosstalk, but how this is regulated remains poorly understood. Here, we show that sorting nexin protein Snx14, an ER-resident protein associated with the cerebellar ataxia SCAR20, localizes to ER-LD contacts following FA treatment, where it promotes LD maturation. Using proximity-based APEX technology and topological dissection, we show that Snx14 accumulates specifically at ER-LD contacts independently of Seipin, where it remains ER-anchored and binds LDs in trans. SNX14^KO cells exhibit perturbed LD morphology, whereas Snx14 over

SUBMITTER: Datta S 

PROVIDER: S-EPMC6446855 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature