Mitotic chromosome alignment ensures mitotic fidelity by promoting interchromosomal compaction during anaphase.

ABSTRACT: Chromosome alignment at the equator of the mitotic spindle is a highly conserved step during cell division; however, its importance to genomic stability and cellular fitness is not understood. Normal mammalian somatic cells lacking KIF18A function complete cell division without aligning chromosomes. These alignment-deficient cells display normal chromosome copy numbers in vitro and in vivo, suggesting that chromosome alignment is largely dispensable for maintenance of euploidy. However, we find that loss of chromosome alignment leads to interchromosomal compaction defects during anaphase, abnormal organization of chromosomes into a single nucleus at mitotic exit, and the formation of micronuclei in vitro and in vivo. These defects slow cell proliferation and are associated with impaired po

SUBMITTER: Fonseca CL 

PROVIDER: S-EPMC6446859 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature