Inhibitors of elastase stimulate murine B lymphocyte differentiation into IgG- and IgA-producing cells.

ABSTRACT: It is well established that dendritic cells and macrophages play a role in antigen presentation to B and T cells and in shaping B and T cell responses via cytokines they produce. We have previously reported that depletion of neutrophils improves the production of mucosal IgA after sublingual immunization with Bacillus anthracis edema toxin as adjuvant. These past studies also demonstrated that an inverse correlation exists between the number of neutrophils and production of IgA by B cells. Using specific inhibitors of elastase, we addressed whether the elastase activity of neutrophil could be the factor that interferes with production of IgA and possibly other immunoglobulin isotypes. We found that murine splenocytes and mesenteric lymph node cells cultured for 5 days in the presence of ne

SUBMITTER: Attia Z 

PROVIDER: S-EPMC6447050 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature