A quantitative approach for measuring the reservoir of latent HIV-1 proviruses.

ABSTRACT: A stable latent reservoir for HIV-1 in resting CD4⁺ T cells is the principal barrier to a cure^1-3. Curative strategies that target the reservoir are being tested^4,5 and require accurate, scalable reservoir assays. The reservoir was defined with quantitative viral outgrowth assays for cells that release infectious virus after one round of T cell activation¹. However, these quantitative outgrowth assays and newer assays for cells that produce viral RNA after activation⁶ may underestimate the reservoir size because one round of activation does not induce all proviruses⁷. Many studies rely on simple assays based on polymerase chain reaction to detect proviral DNA regardless of transcriptional status, but the clinical relevance o

SUBMITTER: Bruner KM 

PROVIDER: S-EPMC6447073 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature