Single-Cell Resolution and Quantitation of Targeted Glucocorticoid Delivery in the Thymus.

ABSTRACT: Glucocorticoids are lipid-soluble hormones that signal via the glucocorticoid receptor (GR), a ligand-dependent transcription factor. Circulating glucocorticoids derive from the adrenals, but it is now apparent that paracrine glucocorticoid signaling occurs in multiple tissues. Effective local glucocorticoid concentrations and whether glucocorticoid delivery can be targeted to specific cell subsets are unknown. We use fluorescence detection of chromatin-associated GRs as biosensors of ligand binding and observe signals corresponding to steroid concentrations over physiological ranges in vitro and in vivo. In the thymus, where thymic epithelial cell (TEC)-synthesized glucocorticoids antagonize negative selection, we find that CD4⁺CD8⁺TCR^hi cells, a small sub

SUBMITTER: Taves MD 

PROVIDER: S-EPMC6447081 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature