Emergent heterogeneity in putative mesenchymal stem cell colonies: Single-cell time lapsed analysis.

ABSTRACT: Bone marrow stromal cells (BMSCs) include a subset of stem cells that are considered promising for developmental studies and therapeutic applications. While it is appreciated generally that BMSC populations can exhibit morphological and functional heterogeneity upon in vitro culture expansion, the potential for heterogeneity within a single colony forming unit-generated ostensibly from a single mother cell-is less explored but is critical to design of both fundamental studies and cell therapy production. Here we observed BMSC colony formation in real time via time lapsed optical imaging and analysis, to quantify whether and how heterogeneity emerged over multiple cell divisions spanning the duration of a typical colony formation unit assay. These analyses demonstrate that such colonies are

SUBMITTER: Rennerfeldt DA 

PROVIDER: S-EPMC6447157 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature