Integration of diffraction phase microscopy and Raman imaging for label-free morpho-molecular assessment of live cells.

ABSTRACT: Label-free quantitative imaging is highly desirable for studying live cells by extracting pathophysiological information without perturbing cell functions. Here, we demonstrate a novel label-free multimodal optical imaging system with the capability of providing comprehensive morphological and molecular attributes of live cells. Our morpho-molecular microscopy (3M) system draws on the combined strength of quantitative phase microscopy (QPM) and Raman microscopy to probe the morphological features and molecular fingerprinting characteristics of each cell under observation. While the commonr-path geometry of our QPM system allows for highly sensitive phase measurement, the Raman microscopy is equipped with dual excitation wavelengths and utilizes the same detection and dispersion system, mak

SUBMITTER: Pandey R 

PROVIDER: S-EPMC6447451 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature