High titer MVA and influenza A virus production using a hybrid fed-batch/perfusion strategy with an ATF system.

ABSTRACT: A cultivation strategy to increase the productivity of Modified Vaccinia Ankara (MVA) virus in high-cell density processes is presented. Based on an approach developed in shake flask cultures, this strategy was established in benchtop bioreactors, comprising the growth of suspension AGE1.CR.pIX cells to high cell densities in a chemically defined medium before infection with the MVA-CR19 virus strain. First, a perfusion regime was established to optimize the cell growth phase. Second, a fed-batch regime was chosen for the initial infection phase to facilitate virus uptake and cell-to-cell spreading. Afterwards, a switch to perfusion enabled the continuous supply of nutrients for the late stages of virus propagation. With maximum infectious titers of 1.0 × 10¹⁰ IU/mL, this hybrid

SUBMITTER: Vazquez-Ramirez D 

PROVIDER: S-EPMC6447503 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature