Live-imaging of Bioengineered Cartilage Tissue using Multimodal Non-linear Molecular Imaging.

ABSTRACT: Coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) are non-linear techniques that allow label-free, non-destructive and non-invasive imaging for cellular and tissue analysis. Although live-imaging studies have been performed previously, concerns that they do not cause any changes at the molecular level in sensitive biological samples have not been addressed. This is important especially for stem cell differentiation and tissue engineering, if CARS/SHG microscopy is to be used as a non-invasive, label-free tool for assessment of the developing neo-tissue. In this work, we monitored the differentiation of human fetal-femur derived skeletal cells into cartilage in three-dimensional cultures using CARS and SHG microscopy and demonstrate the live-imaging of the sa

SUBMITTER: Moura CC 

PROVIDER: S-EPMC6447547 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature