Rapid Detection of Brucella spp. and Elimination of Carryover Using Multiple Cross Displacement Amplification Coupled With Nanoparticles-Based Lateral Flow Biosensor.

ABSTRACT: Brucella spp.is capable of causing disease in a range of animal hosts, and human brucellosis is regarded as a life-threating disease. A novel isothermal amplification technique, termed multiple cross displacement amplification (MCDA), was employed for detecting all Brucella species strains. Brucella-MCDA targets the Bscp31 gene (Brucella species-specific gene) to specifically design a set of 10 primers. The Brucella-MCDA can be coupled with nanoparticles-based lateral flow biosensor (LFB) for highly specific, simple, rapid, and visual detection of Brucella-specific amplicons. Using the protocol, a MCDA amplification followed by 2 min LFB resulted in visualization of DNA products trapped at the LFB test line. Various species of Gram-positive

SUBMITTER: Li S 

PROVIDER: S-EPMC6447675 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature