Activation of Secondary Metabolite Gene Clusters in <i>Streptomyces clavuligerus</i> by the PimM Regulator of <i>Streptomyces natalensis</i>.

ABSTRACT: Expression of non-native transcriptional activators may be a powerful general method to activate secondary metabolites biosynthetic pathways. PAS-LuxR regulators, whose archetype is PimM, activate the biosynthesis of polyene macrolide antifungals and other antibiotics, and have been shown to be functionally preserved across multiple Streptomyces strains. In this work we show that constitutive expression of pimM in Streptomyces clavuligerus ATCC 27064 significantly affected its transcriptome and modifies secondary metabolism. Almost all genes in three secondary metabolite clusters were overexpressed, including the clusters responsible for the biosynthesis of the clinically important clavulanic acid and cephamycin C. In comparison to a control strain, this resulted in 10

SUBMITTER: Martinez-Burgo Y 

PROVIDER: S-EPMC6448028 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature