Mesenchymal stem cells transplanted into spinal cord injury adopt immune cell-like characteristics.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) and their cellular response to various stimuli have been characterized in great detail in culture conditions. In contrast, the cellular response of MSCs in an in vivo setting is still uncharted territory. In this study, we investigated the cellular response of MSCs following transplantation into spinal cord injury (SCI).

Methods

Mouse bone marrow-derived MSCs were transplanted 24 h following severe contusion SCI in mice. As controls, MSCs transplanted to the uninjured spinal cord and non-transplanted MSCs were used. At 7 days post transplantation, the MSCs were isolated from the SCI, and their global transcriptional changes, survival, differentiation, proliferation, apoptosis, and phenotypes were investigated using RNA sequencing, imm

SUBMITTER: Hakim R 

PROVIDER: S-EPMC6448247 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature