Unique metabolic activation of adipose tissue macrophages in obesity promotes inflammatory responses.

ABSTRACT:

Aims/hypothesis

Recent studies have identified intracellular metabolism as a fundamental determinant of macrophage function. In obesity, proinflammatory macrophages accumulate in adipose tissue and trigger chronic low-grade inflammation, that promotes the development of systemic insulin resistance, yet changes in their intracellular energy metabolism are currently unknown. We therefore set out to study metabolic signatures of adipose tissue macrophages (ATMs) in lean and obese conditions.

Methods

F4/80-positive ATMs were isolated from obese vs lean mice. High-fat feeding of wild-type mice and myeloid-specific Hif1α^-/- mice was used to examine the role of hypoxia-inducible factor-1α (HIF-1α) in ATMs part of obese adipose tissue. In vitro, bone marrow-derived macrop

SUBMITTER: Boutens L 

PROVIDER: S-EPMC6448980 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature