Identification and Analysis of Islet Antigen-Specific CD8⁺ T Cells with T Cell Libraries.

ABSTRACT: Type 1 diabetes (T1D) is most likely caused by killing of β cells by autoreactive CD8⁺ T cells. Methods to isolate and identify these cells are limited by their low frequency in the peripheral blood. We analyzed CD8⁺ T cells, reactive with diabetes Ags, with T cell libraries and further characterized their phenotype by CyTOF using class I MHC tetramers. In the libraries, the frequency of islet Ag-specific CD45RO⁺IFN-γ⁺CD8⁺ T cells was higher in patients with T1D compared with healthy control subjects. Ag-specific cells from the libraries of patients with T1D were reactive with ZnT8_186-194, whereas those from healthy control recognized ZnT8_186-194 and other Ags. ZnT8_186-194-reactive CD8⁺ cells

SUBMITTER: Ogura H 

PROVIDER: S-EPMC6449153 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature