Elucidating the transcriptional program of feline injection-site sarcoma using a cross-species mRNA-sequencing approach.

ABSTRACT:

Background

Feline injection-site sarcoma (FISS), an aggressive iatrogenic subcutaneous malignancy, is challenging to manage clinically and little is known about the molecular basis of its pathogenesis. Tumor transcriptome profiling has proved valuable for gaining insights into the molecular basis of cancers and for identifying new therapeutic targets. Here, we report the first study of the FISS transcriptome and the first cross-species comparison of the FISS transcriptome with those of anatomically similar soft-tissue sarcomas in dogs and humans.

Methods

Using high-throughput short-read paired-end sequencing, we comparatively profiled FISS tumors vs. normal tissue samples as well as cultured FISS-derived cell lines vs. skin-derived fibroblasts. We analyzed the mRNA-seq data

SUBMITTER: Wei Q 

PROVIDER: S-EPMC6449919 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature