Bovine Derived <i>in vitro</i> Cultures Generate Heterogeneous Populations of Antigen Presenting Cells.

ABSTRACT: Antigen presenting cells (APC) of the mononuclear phagocytic system include dendritic cells (DCs) and macrophages (Macs) which are essential mediators of innate and adaptive immune responses. Many of the biological functions attributed to these cell subsets have been elucidated using models that utilize in vitro-matured cells derived from common progenitors. However, it has recently been shown that monocyte culture systems generate heterogeneous populations of cells, DCs, and Macs. In light of these findings, we analyzed the most commonly used bovine in vitro-derived APC models and compared them to bona fide DCs. Here, we show that bovine monocyte-derived DCs and Macs can be differentiated on the basis of CD11c and MHC class II (MHCII) expression and that in vitro</

SUBMITTER: Guzman E 

PROVIDER: S-EPMC6450137 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature