Oxidative damage of SP-D abolishes control of eosinophil extracellular DNA trap formation.

ABSTRACT: The asthmatic airways are highly susceptible to inflammatory injury by air pollutants such as ozone (O₃ ), characterized by enhanced activation of eosinophilic granulocytes and a failure of immune protective mechanisms. Eosinophil activation during asthma exacerbation contributes to the proinflammatory oxidative stress by high levels of nitric oxide (NO) production and extracellular DNA release. Surfactant protein-D (SP-D), an epithelial cell product of the airways, is a critical immune regulatory molecule with a multimeric structure susceptible to oxidative modifications. Using recombinant proteins and confocal imaging, we demonstrate here that SP-D directly bound to the membrane and inhibited extracellular DNA trap formation by human and murine eosinophils in a concentration a

SUBMITTER: Yousefi S 

PROVIDER: S-EPMC6450398 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature