BacMam production and crystal structure of nonglycosylated apo human furin at 1.89 A resolution.

ABSTRACT: Furin, also called proprotein convertase subtilisin/kexin 3 (PCSK3), is a calcium-dependent serine endoprotease that processes a wide variety of proproteins involved in cell function and homeostasis. Dysregulation of furin has been implicated in numerous disease states, including cancer and fibrosis. Mammalian cell expression of the furin ectodomain typically produces a highly glycosylated, heterogeneous protein, which can make crystallographic studies difficult. Here, the expression and purification of nonglycosylated human furin using the BacMam technology and site-directed mutagenesis of the glycosylation sites is reported. Nonglycosylated furin produced using this system retains full proteolytic activity indistinguishable from that of the glycosylated protein. Importantly, the nonglyco

SUBMITTER: Pearce KH 

PROVIDER: S-EPMC6450522 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature