Analysis of oral bacterial communities: comparison of HOMINGS with a tree-based approach implemented in QIIME.

ABSTRACT: Background: Molecular taxonomic assignments in oral microbial communities have been made using probe-matching approaches, but never compared to those obtained by more readily accepted tree-based approaches. Objective:  To compare community composition profiles obtained from a probe-matching approach (HOMINGS) to those from a closed-ended tree-based approach (QIIME using the eHOMD database). Design:  HOMINGS and QIIME were used for parallel analysis of ten mock community samples, and of 119 supragingival plaque samples from ecologically unique sites (sound tooth surfaces in healthy subjects, sound tooth surfaces in patients with primary Sjögren's Syndrome, and carious lesions in Sjögren's Syndrome patients). Linear discriminant analysis Effective Size (LEf

SUBMITTER: Palmer RJ 

PROVIDER: S-EPMC6450576 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature