Detection of Lipid Mediators of Inflammation in the Human Tear Film.

ABSTRACT:

Purpose

Lipid mediators of inflammation are a group of signaling molecules produced by various cells under physiological conditions and modulate the inflammatory process during various pathologic conditions. Although eicosanoids and F2-isoprostanes are recognized lipid mediators of inflammation, there is no consensus yet on the extraction and mass spectrometry (MS) method for their analysis in individual human tear samples. Thus, the aim of this study was to develop an optimal method for extraction of lipid mediators of inflammation in the tear film and evaluate MS techniques for their analysis.

Methods

Basal tears were collected from each eye of 19 subjects using glass microcapillaries. Lipid extraction was performed using either varying concentrations of acidified methanol

SUBMITTER: Panthi S 

PROVIDER: S-EPMC6450790 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature