A draft nuclear-genome assembly of the acoel flatworm Praesagittifera naikaiensis.

ABSTRACT:

Background

Acoels are primitive bilaterians with very simple soft bodies, in which many organs, including the gut, are not developed. They provide platforms for studying molecular and developmental mechanisms involved in the formation of the basic bilaterian body plan, whole-body regeneration, and symbiosis with photosynthetic microalgae. Because genomic information is essential for future research on acoel biology, we sequenced and assembled the nuclear genome of an acoel, Praesagittifera naikaiensis.

Findings

To avoid sequence contamination derived from symbiotic microalgae, DNA was extracted from embryos that were free of algae. More than 290x sequencing coverage was achieved using a combination of Illumina (paired-end and mate-pair libraries) and PacBio sequencing. RNA s

SUBMITTER: Arimoto A 

PROVIDER: S-EPMC6451197 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature