L-lactate induces specific genome wide alterations of gene expression in cultured bovine granulosa cells.

ABSTRACT:

Background

Previously, we could show that L-lactate affects cultured bovine granulosa cells (GC) in a specific manner driving the cells into an early pre-ovulatory phenotype. Here we studied genome wide effects in L-lactate-treated GC to further elucidate the underlying mechanisms that are responsible for the L-lactate induced transformation. Cultured estrogen producing GC treated either with L-lactate or vehicle control were subjected to mRNA microarray analysis.

Results

The analysis revealed 487 differentially expressed clusters, representing 461 annotated genes. Of these, 333 (= 318 genes) were identified as up- and 154 (= 143 genes) as down-regulated. As the top up-regulated genes we detected TXNIP, H19 and AHSG as well as our previously established marker transcripts RG

SUBMITTER: Baufeld A 

PROVIDER: S-EPMC6451254 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature