Changes of N6-methyladenosine modulators promote breast cancer progression.

ABSTRACT:

Background

Breast cancer (BC) displays striking genetic, epigenetic and phenotypic diversity. N⁶-methyladenosine (m6A) in mRNA has emerged as a crucial epitranscriptomic modification that controls cancer self-renewal and cell fate. However, the key enzymes of m6A expression and function in human breast carcinogenesis remain unclear.

Methods

The expression of m6A methylases (METTL3, METTL14 and WTAP) and demethylases (FTO and ALKBH5) were analyzed by using ONCOMINE and The Cancer Genome Atlas databases and in 36 pairs of BC and adjacent non-cancerous tissue. The level of m6A in BC patients was detected by ELISA, and the function of m6A was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony formation assay and transwell assa

SUBMITTER: Wu L 

PROVIDER: S-EPMC6451293 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature