Automated morphometry toolbox for analysis of microscopic model organisms using simple bright-field imaging.

ABSTRACT: Model organisms with compact genomes, such as yeast and C aenorhabditis elegans, are particularly useful for understanding organism growth and life/cell cycle. Organism morphology is a critical parameter to measure in monitoring growth and stage in the life cycle. However, manual measurements are both time consuming and potentially inaccurate, due to variations among users and user fatigue. In this paper we present an automated method to segment bright-field images of fission yeast, budding yeast, and C. elegans roundworm, reporting a wide range of morphometric parameters, such as length, width, eccentricity, and others. Comparisons between automated and manual methods on fission yeast reveal good correlation in size values, with the 95% confidence interval lyin

SUBMITTER: Liu G 

PROVIDER: S-EPMC6451328 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature