Performance comparison of four commercially available cytometers using fluorescent, polystyrene, submicron-scale beads.

ABSTRACT: Accurate comparison of flow cytometric data requires an understanding of how the cytometric fingerprint of a sample may vary from instrument to instrument. Key sources of variability include the number, wavelengths, and power of excitation lasers; the number and types of emission detectors; sample-handling systems and options; and whether fixed or dynamic detector voltages are used. To explore this variability, suspensions of three sizes (0.2, 0.5, and 0.8 μm-diameter) of solid, fluorescent, polystyrene beads were prepared. The suspensions were then run on four flow cytometers, keeping instrument settings as consistent as possible. The results are displayed graphically in Figure 3 of the article "Flow cytometry applications in water treatment, distribution, and reuse: A review" (DOI: 10.10

SUBMITTER: Safford HR 

PROVIDER: S-EPMC6451693 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature