Biochemical characterization of three new α-olefin-producing P450 fatty acid decarboxylases with a halophilic property.

ABSTRACT:

Background

The CYP152 family member OleT_JE from Jeotgalicoccus sp. ATCC 8456 has been well-known to catalyze the unusual one-step decarboxylation of free fatty acids towards the formation of terminal alkenes. Efforts to tune up its decarboxylation activity for better production of biological alkenes have been extensively explored via approaches such as site-directed mutagenesis and electron source engineering, but with limited success. To gain more insights into the decarboxylation mechanism and reaction bifurcation (decarboxylation versus hydroxylation), we turned to an alternative approach to explore the natural CYP152 resources for a better variety of enzyme candidates.

Results

We biochemically characterized three new P450 fatty acid decarboxylases inclu

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC6452516 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature