Transcriptome-wide profiling of multiple RNA modifications simultaneously at single-base resolution.

ABSTRACT: The breadth and importance of RNA modifications are growing rapidly as modified ribonucleotides can impact the sequence, structure, function, stability, and fate of RNAs and their interactions with other molecules. Therefore, knowing cellular RNA modifications at single-base resolution could provide important information regarding cell status and fate. A current major limitation is the lack of methods that allow the reproducible profiling of multiple modifications simultaneously, transcriptome-wide and at single-base resolution. Here we developed RBS-Seq, a modification of RNA bisulfite sequencing that enables the sensitive and simultaneous detection of m⁵C, Ψ, and m¹A at single-base resolution transcriptome-wide. With RBS-Seq, m⁵C and m¹A are ac

SUBMITTER: Khoddami V 

PROVIDER: S-EPMC6452723 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature