Non-invasive early detection of acute transplant rejection via nanosensors of granzyme B activity.

ABSTRACT: The early detection of the onset of transplant rejection is critical for the long-term survival of patients. The diagnostic gold standard for detecting transplant rejection involves a core biopsy, which is invasive, has limited predictive power and carries a morbidity risk. Here, we show that nanoparticles conjugated with a peptide substrate specific for the serine protease granzyme B, which is produced by recipient T cells during the onset of acute cellular rejection, can serve as a non-invasive biomarker of early rejection. When administered systemically in mouse models of skin graft rejection, these nanosensors preferentially accumulate in allograft tissue, where they are cleaved by granzyme B, releasing a fluorescent reporter that filters into the recipient's urine. Urinalysis then dis

SUBMITTER: Mac QD 

PROVIDER: S-EPMC6452901 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature