Characterization of Pax3 and Sox10 transgenic Xenopus laevis embryos as tools to study neural crest development.

ABSTRACT: The neural crest is a multipotent population of cells that originates a variety of cell types. Many animal models are used to study neural crest induction, migration and differentiation, with amphibians and birds being the most widely used systems. A major technological advance to study neural crest development in mouse, chick and zebrafish has been the generation of transgenic animals in which neural crest specific enhancers/promoters drive the expression of either fluorescent proteins for use as lineage tracers, or modified genes for use in functional studies. Unfortunately, no such transgenic animals currently exist for the amphibians Xenopus laevis and tropicalis, key model systems for studying neural crest development. Here we describe the generation and characterization of two transg

SUBMITTER: Alkobtawi M 

PROVIDER: S-EPMC6453020 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature