Phosphatidylcholine causes adipocyte-specific lipolysis and apoptosis in adipose and muscle tissues.

ABSTRACT: Phosphatidylcholine (PPC) formula has been therapeutically used to reduce areas of localized fat. However, no single research has been carried out on its effect on a variety of cells in adipose and muscle tissues. Herein, the current study aimed to explore the activity of PPC on different cells in adipose and muscle tissues and to investigate the molecular mechanisms contributing to the effects of PPC on lipolysis and apoptosis. mRNA expression levels of various genes were measured by quantitative real-time PCR. Protein expression levels were observed through Western blotting and cell viability was measured by MTT assay. Lipolysis and caspase 3 activity assay were performed using commercial kits. PPC induces lipolysis and apoptosis in adipocytes (3T3-L1), but not in the other tested cells,

SUBMITTER: Jung TW 

PROVIDER: S-EPMC6453443 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature