Dynamic Variations in Genetic Integrity Accompany Changes in Cell Fate.

ABSTRACT: Pluripotent stem cells hold the potential to form the basis of novel approaches to treatment of disease in vivo as well as to facilitate the generation of models for human disease, providing powerful avenues to discovery of novel diagnostic biomarkers and/or innovative drug regimens in vitro. However, this will require extensive maintenance, expansion, and manipulation of these cells in culture, which raises a concern regarding the extent to which genetic integrity will be preserved throughout these manipulations. We used a mutation reporter (lacI) transgene approach to conduct direct comparisons of mutation frequencies in cell populations that shared a common origin and genetic identity, but were induced to undergo transitions in cell fate between pluripotent and differentiated states, or

SUBMITTER: Chen IC 

PROVIDER: S-EPMC6453492 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature