Optimization of solvent-free enzymatic esterification in eutectic substrate reaction mixture.

ABSTRACT: The Candida rugosa lipase catalyzed esterification of (-)-menthol and lauric acid (LA) was studied in a eutectic mixture formed by both substrates((-)-menthol:LA 3:1, mol/mol). No additional reaction solvent was necessary, since the (-)-menthol:LA deep eutectic solvent (DES) acts as combined reaction medium and substrate pool. Therefore, the esterification is conducted under solvent-free conditions. The thermodynamic water activity (a_w) was identified as a key parameter affecting the esterification performance in the (-)-menthol:LA DES. A response surface methodology was applied to optimize the esterification conditions in terms a_w, amount of C. rugosa lipase (m_CRL) and reaction temperature. Under the optimized reaction conditions (a_w

SUBMITTER: Patzold M 

PROVIDER: S-EPMC6453777 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature