A novel hepatitis B virus recombinant genotype D4/E identified in a South African population.

ABSTRACT:

Background

Genetic diversity is a characteristic trait of the hepatitis B virus (HBV) and has been associated with different clinical outcomes. In South Africa, HBV infection is a major public health concern. Most HBV infections are caused by genotype A strains. However rare cases of infection with HBV genotype D have been reported. The purpose of this study was to investigate the molecular characteristics of a rare HBV subgenotype D4 isolate.

Methods

The full-length genome of isolate ZADGM6964 was amplified in a one-step polymerase chain reaction. The amplified product was purified and cloned into a pGEM®-T Easy Vector System to investigate the genetic diversity of the viral quasi-populations. The primary isolate and clones were then directly sequenced and analysed using an

SUBMITTER: Matlou MK 

PROVIDER: S-EPMC6453802 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature