A metabolomic study of the effect of Candida albicans glutamate dehydrogenase deletion on growth and morphogenesis.

ABSTRACT: There are two glutamate dehydrogenases in the pathogenic fungus Candida albicans. One is an NAD⁺-dependent glutamate dehydrogenase (GDH2) and the other is an NADPH-dependent glutamate dehydrogenase (GDH3). These two enzymes are part of the nitrogen and nicotinate/nicotinamide metabolic pathways, which have been identified in our previous studies as potentially playing an important role in C. albicans morphogenesis. In this study, we created single gene knockout mutants of both dehydrogenases in order to investigate whether or not they affect the morphogenesis of C. albicans. The GDH genes were deleted and the phenotypes of the knockout mutants were studied by growth characterisation, metabolomics, isotope labelling experiments, and by qu

SUBMITTER: Han TL 

PROVIDER: S-EPMC6453907 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature