Changing surface grafting density has an effect on the activity of immobilized xylanase towards natural polysaccharides.

ABSTRACT: Enzymes are involved in various types of biological processes. In many cases, they are part of multi-component machineries where enzymes are localized in close proximity to each-other. In such situations, it is still not clear whether inter-enzyme spacing actually plays a role or if the colocalization of complementary activities is sufficient to explain the efficiency of the system. Here, we focus on the effect of spatial proximity when identical enzymes are immobilized onto a surface. By using an innovative grafting procedure based on the use of two engineered protein fragments, Jo and In, we produce model systems in which enzymes are immobilized at surface densities that can be controlled precisely. The enzyme used is a xylanase that participates to the hydrolysis of plant cell wall poly

SUBMITTER: Montanier CY 

PROVIDER: S-EPMC6453946 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature