Uncoupling of in-vitro identity of embryonic limb derived skeletal progenitors and their in-vivo bone forming potential.

ABSTRACT: The healing of large bone defects remains a major unmet medical need. Our developmental engineering approach consists of the in vitro manufacturing of a living cartilage tissue construct that upon implantation forms bone by recapitulating an endochondral ossification process. Key to this strategy is the identification of the cells to produce such cartilage intermediates efficiently. We applied a cell selection strategy based on published skeletal stem cell markers using mouse embryonic limb cartilage as cell source and analysed their potential to form bone in an in vivo ectopic assay. FGF2 supplementation to the culture media for expansion blocked dedifferentiation of the embryonic cartilage cells in culture and enriched for stem cells and progenitors as quantified using the recently publi

SUBMITTER: Verbeeck L 

PROVIDER: S-EPMC6453955 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature