Sec14l3 potentiates VEGFR2 signaling to regulate zebrafish vasculogenesis.

ABSTRACT: Vascular endothelial growth factor (VEGF) regulates vasculogenesis by using its tyrosine kinase receptors. However, little is known about whether Sec14-like phosphatidylinositol transfer proteins (PTP) are involved in this process. Here, we show that zebrafish sec14l3, one of the family members, specifically participates in artery and vein formation via regulating angioblasts and subsequent venous progenitors' migration during vasculogenesis. Vascular defects caused by sec14l3 depletion are partially rescued by restoration of VEGFR2 signaling at the receptor or downstream effector level. Biochemical analyses show that Sec14l3/SEC14L2 physically bind to VEGFR2 and prevent it from dephosphorylation specifically at the Y¹¹⁷⁵ site by peri-membrane tyrosine phosphatase PTP1B, therefo

SUBMITTER: Gong B 

PROVIDER: S-EPMC6453981 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature