NF-κB Signaling and IL-4 Signaling Regulate SATB1 Expression via Alternative Promoter Usage During Th2 Differentiation.

ABSTRACT: SATB1 is a genome organizer protein that is expressed in a lineage specific manner in CD4⁺ T-cells. SATB1 plays a crucial role in expression of multiple genes throughout the thymic development and peripheral differentiation of T cells. Although SATB1 function has been subjected to intense investigation, regulation of SATB1 gene expression remains poorly understood. Analysis of RNA-seq data revealed multiple transcription start sites at the upstream regulatory region of SATB1. We further demonstrated that SATB1 gene is expressed via alternative promoters during T-helper (Th) cell differentiation. The proximal promoter "P1" is used more by the naïve and activated CD4⁺ T-cells whereas the middle "P2" and the distal "P3" promoters are used at a signif

SUBMITTER: Khare SP 

PROVIDER: S-EPMC6454056 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature