Identification of Androgen Receptor Modulators in a Prostate Cancer Cell Line Microarray Compendium.

ABSTRACT: High-throughput transcriptomic (HTTr) technologies are increasingly being used to screen environmental chemicals in vitro to identify molecular targets and provide mechanistic context for regulatory testing. Here, we describe the development and validation of a novel gene expression biomarker to identify androgen receptor (AR)-modulating chemicals using a pattern matching method. Androgen receptor biomarker genes were identified by their consistent expression after exposure to 4 AR agonists and 4 AR antagonists and included only those genes that were regulated by AR. The 51 gene biomarker was evaluated as a predictive tool using the fold-change, rank-based Running Fisher algorithm. Using 158 comparisons from cells treated with 95 chemicals, the biomarker gave balanced accuracies for predic

SUBMITTER: Rooney JP 

PROVIDER: S-EPMC6454424 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature