Whole-genome analysis reveals the evolution and transmission of an MDR DH/NAP11/106 Clostridium difficile clone in a paediatric hospital.

ABSTRACT:

Background

Clostridium difficile strain DH/NAP11/106, a relatively antibiotic-susceptible strain, is now the most common cause of C. difficile infection (CDI) among adults in the USA.

Objectives

To identify mechanisms underlying the evolution and transmission of an MDR DH/NAP11/106 clone.

Methods

WGS (Illumina MiSeq), restriction endonuclease analysis (REA) and antibiotic susceptibility testing were performed on 134 C. difficile isolates collected from paediatric patients with CDI over a 2 year period.

Results

Thirty-one of 134 (23%) isolates were REA group DH. Pairwise single-nucleotide variant (SNV) analyses identified a DH clone causing seven instances of CDI in two patients. During the 337 days between the first and second CDI, Patient 1 (P1) received 313 d

SUBMITTER: Kociolek LK 

PROVIDER: S-EPMC6454525 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature