Loss of exosomal miR-3188 in cancer-associated fibroblasts contributes to HNC progression.

ABSTRACT:

Background

Head and neck cancer (HNC) is one of the most common deadly diseases worldwide. An increasing number of studies have recently focused on the malignant functions of cancer-associated fibroblasts (CAFs) in numerous cancers. However, the underlying mechanisms by which CAF-derived exosomes promote tumor progression need to be further elucidated. This study aims to determine whether the loss of specific miRNAs in CAF-derived exosomes may be involved in the malignant transformation of HNC.

Methods

MiRNA array and real-time PCR assays were used to analyze the differential expression of miRNAs in exosomes from normal fibroblasts (NFs) and CAFs. Cell proliferation, EdU incorporation, colony formation, apoptosis, cell cycle distribution and xenograft assays were performed t

SUBMITTER: Wang X 

PROVIDER: S-EPMC6454737 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature